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For the acute toxicity test, the 96 h LC50 level was measured and was predicted to be as low as 0.77 ppm (around 28 nM) by a curve-fitting method after the zebrafish embryos were treated with different doses of Cu 2+ , from 10 −2 to 10 4 ppm (Figure 3B)

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doi: 10.1002/jcp.29013 227 LiuDFangYRaoYTanWZhouWWuXet al