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Early research in the 1970s and 1980s focused on characterizing GHK-Cu's biochemical properties: Identification of the peptide's amino acid sequence (glycine-histidine-lysine) Demonstration that GHK-Cu stimulates collagen synthesis in fibroblasts Evidence that GHK-Cu attracts immune cells (macrophages, mast cells) to wound sites Documentation of GHK-Cu's role in copper transport and metabolism This early work established GHK-Cu as a significant biological molecule with applications in tissue repair